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Western blot analysis of extracts of various cell lines using MESDC2 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using MESDC2 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using MESDC2 Polyclonal Antibody at dilution of 1:3000.

MESDC2 Polyclonal Antibody

E-AB-64868
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    MESDC2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    MESD (Mesoderm Development LRP Chaperone) is a Protein Coding gene. Diseases associated with MESD include Osteogenesis Imperfecta, Type Xx and Epilepsy, Nocturnal Frontal Lobe, 2. Among its related pathways are Wnt / Hedgehog / Notch.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MESDC2/MESD using anti-MESDC2/MESD antibody (A32230-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MESDC2/MESD antigen affinity purified polyclonal antibody (Catalog # A32230-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MESDC2/MESD at approximately 24 kDa. The expected band size for MESDC2/MESD is at 26 kDa.
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