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ProductsBack/Mesothelin (C-3): m-IgGkappa BP-HRP Bundle

Mesothelin (C-3): m-IgGkappa BP-HRP Bundle

SC-521517
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMSLN
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Mesothelin (C-3): m-IgGkappa BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10232
  • Target name
    MSLN
  • Target description
    mesothelin
  • Target synonyms
    MPF, SMRP, mesothelin, CAK1 antigen, megakaryocyte potentiating factor, pre-pro-megakaryocyte-potentiating factor, soluble MPF mesothelin related protein
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mesothelin using anti-Mesothelin antibody (PB9287). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human Mesothelin Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mesothelin antigen affinity purified polyclonal antibody (Catalog # PB9287) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mesothelin at approximately 43KD. The expected band size for Mesothelin is at 43KD.
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