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Metabotropic Glutamate Receptor 2 Antibody (YA3218, Rabbit)

HY-P83473
MedChem Express
TargetGRM2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Metabotropic Glutamate Receptor 2 Antibody (YA3218, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2912
  • Target name
    GRM2
  • Target description
    glutamate metabotropic receptor 2
  • Target synonyms
    GLUR2, GPRC1B, MGLUR2, mGlu2, metabotropic glutamate receptor 2, glutamate receptor homolog, glutamate receptor, metabotropic 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Metabotropic Glutamate Receptor 2 Antibody (YA3218) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3218), targeting Metabotropic Glutamate Receptor 2, with a predicted molecular weight of 96 kDa (observed band size: 99 kDa). Metabotropic Glutamate Receptor 2 Antibody (YA3218) can be used for WB, IHC-P experiment in human, mouse background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRM2 using anti-GRM2 antibody (A06123-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRM2 antigen affinity purified polyclonal antibody (Catalog # A06123-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRM2 at approximately 100kDa and >200 kDa. The expected band size for GRM2 is at 96 kDa.
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