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ProductsBack/Methylthioadenosine phosphorylase Recombinant Antbody Kit (KD-Validated)

Methylthioadenosine phosphorylase Recombinant Antbody Kit (KD-Validated)

BSM-90196R
Bioss
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetMTAP
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Overview

  • Supplier
    Bioss
  • Product Name
    Methylthioadenosine phosphorylase Recombinant Antbody Kit (KD-Validated)
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IC(1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID4507
  • Target name
    MTAP
  • Target description
    methylthioadenosine phosphorylase
  • Target synonyms
    BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus, S-methyl-5'-thioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, MTA phosphorylase, MTAPase, MeSAdo phosphorylase, epididymis luminal protein 249, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13126
  • Protein Name
    S-methyl-5'-thioadenosine phosphorylase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MTAP using anti-MTAP antibody (A05448-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTAP antigen affinity purified polyclonal antibody (Catalog # A05448-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTAP at approximately 31 kDa. The expected band size for MTAP is at 31 kDa.
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