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Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, Mouse liver tissue, Mouse kidney tissue All lanes: MTAP antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 32, 39, 37, 31, 34, 33, 27 kDa Observed band size: 32, 39 kDa
Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, Mouse liver tissue, Mouse kidney tissue All lanes: MTAP antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 32, 39, 37, 31, 34, 33, 27 kDa Observed band size: 32, 39 kDa
Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, Mouse liver tissue, Mouse kidney tissue All lanes: MTAP antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 32, 39, 37, 31, 34, 33, 27 kDa Observed band size: 32, 39 kDa

MTAP Antibody

CSB-PA622639LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetMTAP
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Overview

  • Supplier
    Cusabio
  • Product Name
    MTAP Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4507
  • Target name
    MTAP
  • Target description
    methylthioadenosine phosphorylase
  • Target synonyms
    5'-methylthioadenosine phosphorylase; BDMF; c86fus; DMSFH; DMSMFH; epididymis luminal protein 249; epididymis secretory sperm binding protein; HEL-249; LGMBF; MeSAdo phosphorylase; MSAP; MTA phosphorylase; MTAPase; S-methyl-5'-thioadenosine phosphorylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13126
  • Protein Name
    S-methyl-5'-thioadenosine phosphorylase
  • Scientific Description
    Catalyzes the reversible phosphorylation of S-methyl-5-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MTAP using anti-MTAP antibody (A05448-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTAP antigen affinity purified polyclonal antibody (Catalog # A05448-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTAP at approximately 31 kDa. The expected band size for MTAP is at 31 kDa.
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