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ProductsBack/MFF antibody [HL1312]
U87-MG whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MFF antibody [HL1312] (GTX636730) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
U87-MG whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MFF antibody [HL1312] (GTX636730) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
U87-MG whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MFF antibody [HL1312] (GTX636730) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MFF antibody [HL1312]

GTX636730
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMFF
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Overview

  • Supplier
    GeneTex
  • Product Name
    MFF antibody [HL1312]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1312
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID56947
  • Target name
    MFF
  • Target description
    mitochondrial fission factor
  • Target synonyms
    C2orf33, EMPF2, GL004, mitochondrial fission factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZY8
  • Protein Name
    Mitochondrial fission factor
  • Scientific Description
    This is a nuclear gene encoding a protein that functions in mitochondrial and peroxisomal fission. The encoded protein recruits dynamin-1-like protein (DNM1L) to mitochondria. There are multiple pseudogenes for this gene on chromosomes 1, 5, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MFF using anti-MFF antibody (A02563-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human MOLT-4 whole clel lysates, Lane 5: human HEL whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFF antigen affinity purified polyclonal antibody (Catalog # A02563-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MFF at approximately 26 kDa. The expected band size for MFF is at 26 kDa.
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