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MFGE8 Antibody Pair

CSB-EAP07974
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Goat, Human, Porcine
TargetMFGE8
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Overview

  • Supplier
    Cusabio
  • Product Name
    MFGE8 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.5ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID4240
  • Target name
    MFGE8
  • Target description
    milk fat globule EGF and factor V/VIII domain containing
  • Target synonyms
    BA46; breast epithelial antigen BA46; EDIL1; HMFG; hP47; HsT19888; lactadherin; medin; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08431
  • Protein Name
    Lactadherin
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Goat, Human, Porcine
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of Milk Fat Globule 1/MFGE8 using anti-Milk Fat Globule 1/MFGE8 antibody (A02518-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human milk 5 microl, Lane 2: human milk 5 microl, Lane 3: human milk 2.5 microl, Lane 4: human milk 2.5 microl. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Milk Fat Globule 1/MFGE8 antigen affinity purified polyclonal antibody (Catalog # A02518-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Milk Fat Globule 1/MFGE8 at approximately 43 kDa. The expected band size for Milk Fat Globule 1/MFGE8 is at 43 kDa.
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