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mGluR1 Antibody (YA3195, Rabbit)

HY-P83450
MedChem Express
TargetGRM1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    mGluR1 Antibody (YA3195, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2911
  • Target name
    GRM1
  • Target description
    glutamate metabotropic receptor 1
  • Target synonyms
    glutamate receptor, metabotropic 1; GPRC1A; metabotropic glutamate receptor 1; MGLU1; MGLUR1; PPP1R85; protein phosphatase 1, regulatory subunit 85; SCA44; SCAR13
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    mGluR1 Antibody (YA3195) is a non-conjugated IgG antibody, targeting mGluR1, with a predicted molecular weight of 132 kDa (observed band size: 101 kDa). mGluR1 Antibody (YA3195) can be used for WB experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of mGluR1/GRM1 using anti-mGluR1/GRM1 antibody (A03049-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mGluR1/GRM1 antigen affinity purified polyclonal antibody (Catalog # A03049-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for mGluR1/GRM1 at approximately 132KD. The expected band size for mGluR1/GRM1 is at 132KD.
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