WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2]. Dilution : 1:1000
![WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX27045/GTX27045_1305_WB_w_23060722_763.webp "WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2].")
![ICC/IF analysis of HeLa cells using GTX27045 MGMT antibody [MT 23.2]. Green : primary antibody Blue : Hoechst 33258 Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX27045/GTX27045_163_ICC-IF_w_23060722_251.webp "ICC/IF analysis of HeLa cells using GTX27045 MGMT antibody [MT 23.2]. Green : primary antibody Blue : Hoechst 33258 Dilution : 1:50")
![ICC/IF analysis of MCF-7 cells using GTX27045 MGMT antibody [MT 23.2]. Green : primary antibody Red : Actin Blue : DAPI Dilution : 1:200 Fixation : 10% formalin Permibilization : 0.5% Triton-X100](https://www.genetex.com/upload/website/prouct_img/normal/GTX27045/GTX27045_164_ICC-IF_w_23060722_434.webp "ICC/IF analysis of MCF-7 cells using GTX27045 MGMT antibody [MT 23.2]. Green : primary antibody Red : Actin Blue : DAPI Dilution : 1:200 Fixation : 10% formalin Permibilization : 0.5% Triton-X100")
![IHC-P analysis of human kidney tissue using GTX27045 MGMT antibody [MT 23.2]. Dilution : 1:2000 Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)](https://www.genetex.com/upload/website/prouct_img/normal/GTX27045/GTX27045_527_IHC-P_w_23060722_809.webp "IHC-P analysis of human kidney tissue using GTX27045 MGMT antibody [MT 23.2]. Dilution : 1:2000 Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)")
![FACS (Intracellular staining) analysis of Jurkat cells using GTX27045 MGMT antibody [MT 23.2]. Blue : Primary antibody Orange : isotype control Dilution : 1 microg/mL Fixation : 4% PFA Permibilization : 0.1% saponin](https://www.genetex.com/upload/website/prouct_img/normal/GTX27045/GTX27045_90_FACS_w_23060722_714.webp "FACS (Intracellular staining) analysis of Jurkat cells using GTX27045 MGMT antibody [MT 23.2]. Blue : Primary antibody Orange : isotype control Dilution : 1 microg/mL Fixation : 4% PFA Permibilization : 0.1% saponin")
![FACS (Intracellular staining) analysis of Raji cells using GTX27045 MGMT antibody [MT 23.2]. Blue : Primary antibody Orange : isotype control Dilution : 2 microg/mL Fixation : 4% PFA Permibilization : 0.1% saponin](https://www.genetex.com/upload/website/prouct_img/normal/GTX27045/GTX27045_92_FACS_w_23060722_979.webp "FACS (Intracellular staining) analysis of Raji cells using GTX27045 MGMT antibody [MT 23.2]. Blue : Primary antibody Orange : isotype control Dilution : 2 microg/mL Fixation : 4% PFA Permibilization : 0.1% saponin")
WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2]. Dilution : 1:1000
MGMT antibody [MT 23.2]
GTX27045
ApplicationsFlow Cytometry, ImmunoFluorescence, Mass Spectrometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetMGMT
Overview
- SupplierGeneTex
- Product NameMGMT antibody [MT 23.2]
- Delivery Days Customer9
- Antibody SpecificityWe do not recommend use of this product for Mouse.
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:500. IHC-P: 1:500 - 1:2000. FACS: 1 - 2 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Mass Spectrometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDMT 23.2
- Concentration1 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID4255
- Target nameMGMT
- Target descriptionO-6-methylguanine-DNA methyltransferase
- Target synonyms6-O-methylguanine-DNA methyltransferase; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase
- HostMouse
- IsotypeIgG2b
- Protein IDP16455
- Protein NameMethylated-DNA--protein-cysteine methyltransferase
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- miRNA array screening reveals cooperative MGMT-regulation between miR-181d-5p and miR-409-3p in glioblastoma. Khalil S et al., 2016 May 10, OncotargetRead more
- Mismatch repair-dependent processing of methylation damage gives rise to persistent single-stranded gaps in newly replicated DNA. Mojas N et al., 2007 Dec 15, Genes DevRead more