More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/MGMT antibody [MT 23.2]
WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2]. Dilution : 1:1000
WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2]. Dilution : 1:1000
WB analysis of MCF-7 cell lysate using GTX27045 MGMT antibody [MT 23.2]. Dilution : 1:1000

MGMT antibody [MT 23.2]

GTX27045
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Mass Spectrometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMGMT
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    MGMT antibody [MT 23.2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 1:500. IHC-P: 1:500 - 1:2000. FACS: 1 - 2 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Mass Spectrometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MT 23.2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4255
  • Target name
    MGMT
  • Target description
    O-6-methylguanine-DNA methyltransferase
  • Target synonyms
    methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, O6-methylguanine-DNA methyltransferase, methylguanine-DNA methyltransferase
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP16455
  • Protein Name
    Methylated-DNA--protein-cysteine methyltransferase
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Khalil S, Fabbri E, Santangelo A, et al. miRNA array screening reveals cooperative MGMT-regulation between miR-181d-5p and miR-409-3p in glioblastoma. Oncotarget. 2016,7(19):28195-206. doi: 10.18632/oncotarget.8618
    Read this paper
  • Mojas N, Lopes M, Jiricny J. Mismatch repair-dependent processing of methylation damage gives rise to persistent single-stranded gaps in newly replicated DNA. Genes Dev. 2007,21(24):3342-55.
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of Jurkat cells using MGMT Polyclonal Antibody
Product group Antibodies
Cusabio
MGMT AntibodyCSB-PA003238
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetMGMT
  • SizePrice
Product group Antibodies
GeneTex
MGMT antibodyGTX110551
ApplicationsImmunoPrecipitation, Western Blot
TargetMGMT
  • SizePrice
WB analysis of HepG2 cell lysate using GTX64402 MGMT antibody. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
MGMT antibodyGTX64402
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetMGMT
  • SizePrice
WB analysis of various samples using GTX66683 MGMT antibody. Lane1 : L02 whole cell lysate(40ug) Lane2 : A549 whole cell lysate(40ug) Lane3 : PC3 whole cell lysate(40ug) Lane4 : CT26 whole cell lysate(40ug) Lane5 : PC12 whole cell lysate(40ug) Dilution : 1:500
Product group Antibodies
GeneTex
MGMT antibodyGTX66683
ApplicationsWestern Blot
TargetMGMT
  • SizePrice
Product group Antibodies
MedChem Express
MGMT Antibody (YA293, Rabbit)HY-P80224
TargetMGMT
  • SizePrice
Anti-MGMT Antibody
Product group Antibodies
Antibodies.com
Anti-MGMT AntibodyA43686
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
RayBiotech
Anti-MGMT Antibody144-00693
ApplicationsImmunoFluorescence, Western Blot
TargetMGMT
  • SizePrice
Figure 1. Western blot analysis of MGMT using anti-MGMT antibody (PB9266). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGMT antigen affinity purified polyclonal antibody (Catalog # PB9266) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGMT at approximately 22 kDa. The expected band size for MGMT is at 22 kDa.
Product group Antibodies
Boster Bio
Anti-MGMT Antibody Picoband(r)PB9266-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetMGMT
  • SizePrice
Product group Antibodies
Nordic-MUbio
Rabbit anti Human MGMTX2730P
ApplicationsWestern Blot, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Biomatik
O-6-Methylguanine DNA Methyltransferase (MGMT) Polyclonal AntibodyCAU24824
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetMGMT
  • SizePrice