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WB analysis of K562 cell lysate (35ug/lane) using GTX53672 MIB1 antibody.
WB analysis of K562 cell lysate (35ug/lane) using GTX53672 MIB1 antibody.
WB analysis of K562 cell lysate (35ug/lane) using GTX53672 MIB1 antibody.

MIB1 antibody

GTX53672
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMIB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    MIB1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 1:20-1:100. IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57534
  • Target name
    MIB1
  • Target description
    MIB E3 ubiquitin protein ligase 1
  • Target synonyms
    DAPK-interacting protein 1; DIP1; DIP-1; E3 ubiquitin-protein ligase MIB1; LVNC7; MIB; mindbomb E3 ubiquitin protein ligase 1; RING-type E3 ubiquitin transferase MIB1; ubiquitin ligase mind bomb; zinc finger ZZ type with ankyrin repeat domain protein 2; ZZANK2; ZZZ6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86YT6
  • Protein Name
    E3 ubiquitin-protein ligase MIB1
  • Scientific Description
    This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). [provided by RefSeq, Jun 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mib1 using anti-Mib1 antibody (A01387-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mib1 antigen affinity purified polyclonal antibody (Catalog # A01387-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mib1 at approximately 110 kDa. The expected band size for Mib1 is at 110 kDa.
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