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Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-1853R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 51kD. Observed band size: 51kD.
Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-1853R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 51kD. Observed band size: 51kD.
Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-1853R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 51kD. Observed band size: 51kD.

MMP11 Polyclonal Antibody

BS-1853R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMMP11
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MMP-11 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4320
  • Target name
    MMP11
  • Target description
    matrix metallopeptidase 11
  • Target synonyms
    matrix metallopeptidase 11 (stromelysin 3); SL-3; ST3; STMY3; stromelysin III; stromelysin-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24347
  • Protein Name
    Stromelysin-3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MMP11 using anti-MMP11 antibody (A04490-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat spleen tissue lysate, lane 2: MM231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP11 antigen affinity purified polyclonal antibody (Catalog # A04490-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP11 at approximately 55KD. The expected band size for MMP11 is at 55KD.
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