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Western Blot analysis of A549 cells using MMP-11 Polyclonal Antibody
Western Blot analysis of A549 cells using MMP-11 Polyclonal Antibody
Western Blot analysis of A549 cells using MMP-11 Polyclonal Antibody

MMP11 Antibody

CSB-PA003254
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMMP11
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Overview

  • Supplier
    Cusabio
  • Product Name
    MMP11 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4320
  • Target name
    MMP11
  • Target description
    matrix metallopeptidase 11
  • Target synonyms
    SL-3, ST3, STMY3, stromelysin-3, matrix metallopeptidase 11 (stromelysin 3), stromelysin III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24347
  • Protein Name
    Stromelysin-3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MMP11 using anti-MMP11 antibody (A04490-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat spleen tissue lysate, lane 2: MM231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP11 antigen affinity purified polyclonal antibody (Catalog # A04490-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP11 at approximately 55KD. The expected band size for MMP11 is at 55KD.
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