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ProductsBack/MMP16 Antibody - C-terminal region (OAAB10013)

MMP16 Antibody - C-terminal region (OAAB10013)

OAAB10013
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMMP16
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    MMP16 Antibody - C-terminal region (OAAB10013)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Gene ID4325
  • Target name
    MMP16
  • Target description
    matrix metallopeptidase 16
  • Target synonyms
    C8orf57, MMP-X2, MT-MMP2, MT-MMP3, MT3-MMP, matrix metalloproteinase-16, MMP-16, MT-MMP 3, MT3MMP, MTMMP3, Putative transmembrane protein C8orf57, matrix metallopeptidase 16 (membrane-inserted), membrane-type matrix metalloproteinase 3, membrane-type-3 matrix metalloproteinase
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MMP16 using anti-MMP16 antibody (A05065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse intestine tissue lysates, Lane 2: human SMMC-7721 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP16 antigen affinity purified polyclonal antibody (Catalog # A05065) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP16 at approximately 53KD. The expected band size for MMP16 is at 53KD.
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