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MMP8 Antibody (clone 206, Preservative Free)

LS-C819092
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetMMP8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MMP8 Antibody (clone 206, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA(Cap): This antibody will detect Human MMP-8 / MMP8 in ELISA pair set. In a sandwich ELISA, it can be used as a capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Applications
    ELISA
  • Applications Supplier
    ELISA ELISA(Cap): This antibody will detect Human MMP-8 / MMP8 in ELISA pair set. In a sandwich ELISA, it can be used as a capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    206
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4317
  • Target name
    MMP8
  • Target description
    matrix metallopeptidase 8
  • Target synonyms
    CLG1, HNC, MMP-8, PMNL-CL, neutrophil collagenase, PMN leukocyte collagenase, PMNL collagenase, collagenase 2, matrix metalloproteinase 8 (neutrophil collagenase), matrix metalloproteinase-8
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of MMP8 using anti-MMP8 antibody (PB9726). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. Lane 1: K562 Whole Cell Lysate, Lane 2: JURKAT Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP8 antigen affinity purified polyclonal antibody (Catalog # PB9726) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP8 at approximately 60KD. The expected band size for MMP8 is at 53KD.
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