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ProductsBack/Moesin (MSN) Mouse Monoclonal Antibody [Clone ID: 2C12]
Western blot analysis using MSN antibody against human MSN (AA: 292-491) recombinant protein (Expected MW is 49.2 kDa).
Western blot analysis using MSN antibody against human MSN (AA: 292-491) recombinant protein (Expected MW is 49.2 kDa).
Western blot analysis using MSN antibody against human MSN (AA: 292-491) recombinant protein (Expected MW is 49.2 kDa).

Moesin (MSN) Mouse Monoclonal Antibody [Clone ID: 2C12]

AM06738PU-N
OriGene
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey
TargetMSN
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Moesin (MSN) Mouse Monoclonal Antibody [Clone ID: 2C12]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2C12
  • Gene ID4478
  • Target name
    MSN
  • Target description
    moesin
  • Target synonyms
    HEL70, IMD50, moesin, epididymis luminal protein 70, membrane-organizing extension spike protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP26038
  • Protein Name
    Moesin
  • Scientific Description
    Moesin (MSN) mouse monoclonal antibody, clone 2C12, Purified
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MSN using anti-MSN antibody (A00766-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSN antigen affinity purified polyclonal antibody (Catalog # A00766-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSN at approximately 78KD. The expected band size for MSN is at 66KD.
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