Mouse anti Collagen Type IV

Nordic-MUbio

Mouse anti Collagen Type IV

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1043 recognizes type IV collagen in the extracellular - basement membranes of normal and pathological human tissues.

1043 is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 . For immunohistochemistry on paraffin sections the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration; 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Flow Cytometry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting

Primary antibodies

Research Use Only

Monoclonal

1043

COL4A2

collagen type IV alpha 2 chain

BSVD2; canstatin; collagen alpha-2(IV) chain; ICH; POREN2

Mouse

IgG1

Collagen alpha-2(IV) chain

1043 is a mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with purified human type IV collagen. The immunogen has been isolated from human placenta.

Human

Human

12352203