Mouse anti Cytokeratin 18 / Keratin K18
MUB0326P
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityCanine, Chicken, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Zebra Fish
TargetKRT18
Overview
- SupplierNordic-MUbio
- Product NameMouse anti Cytokeratin 18 / Keratin K18
- Delivery Days Customer7
- Antibody SpecificityRGE53 reacts exclusively with Cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiRatory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.
- Application Supplier NoteRGE53 is suitable for immunoblotting, immunocytochemistry on acetone fixed cells, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
- ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- Applications SupplierFlow Cytometry;Immunocytochemistry;Immunohistochemistry (frozen);Western Blotting
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRGE53
- Gene ID3875
- Target nameKRT18
- Target descriptionkeratin 18
- Target synonymscell proliferation-inducing gene 46 protein; CK-18; CYK18; cytokeratin 18; K18; keratin 18, type I; keratin, type I cytoskeletal 18
- HostMouse
- IsotypeIgG1
- Protein IDP05783
- Protein NameKeratin, type I cytoskeletal 18
- SourceRGE53 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal preparation of HeLa cells.
- ReactivityCanine, Chicken, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Zebra Fish
- Reactivity SupplierCanine;Chicken;Hamster;Human;Mouse;Rabbit;Rat;Swine;Zebrafish
- UNSPSC12352203
References
- 1. Ramaekers, F., Huysmans, A., Moesker, O., Kant, A., Jap, P., Herman, C., and Vooijs, P. (1983). Monoclonal antibody to Keratin filaments, specific for glandular epithelia and their tumors. Use in surgical pathology, Lab Invest 49, 353-61. 2. Ramaekers, F. C., Puts, J. J., Moesker, O., Kant, A., Huysmans, A., Haag, D., Jap, P. H., Herman, C. J., and Vooijs, G. P. (1983). Antibodies to intermediate filament proteins in the immunohistochemical identifiCation of Human tumours: an overview, Histochem J 15, 691-713. 3. Puts, J. J., Moesker, O., Kenemans, P., Vooijs, G. P., and Ramaekers, F. C. (1985). Expression of Cytokeratins in early neoplastic epithelial lesions of the uterine cervix, Int J Gynecol Pathol 4, 300-13. 4. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to Keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55. 5. Raats, J. M., Pieper, F. R., Vree Egberts, W. T., Verrijp, K. N., Ramaekers, F. C., and Bloemendal, H. (1990). Assembly of amino-terminally deleted desmin in vimentin-free cells, J Cell Biol 111, 1971-85. 6. Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of Keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68. 7. Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Link, M., Lane, B., Leigh, I., Schijf, C., and Vooijs, P. (1992). Keratin expression in cervical cancer, Am J Pathol 141, 497-511. 8. van Leenders, G., Dijkman, H., Hulsbergen-van de Kaa, C., Ruiter, D., and Schalken, J. (2000). Demonstration of intermediate cells during Human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy, Lab Invest 80, 1251-8.