Mouse anti Cytokeratin 18 / Keratin K18

Nordic-MUbio

Mouse anti Cytokeratin 18 / Keratin K18

7

DE-K18 reacts exclusively with Cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiRatory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.

DE-K18 is useful for immunocytochemistry, immunoblotting, flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues. For staining on paraffin-embedded tissues pretreatment with 0,1% pepsin in 0.1 N HCl 30 min at room temperature is required. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Flow Cytometry;Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting

Primary antibodies

Research Use Only

Monoclonal

DE-K18

KRT18

keratin 18

cell proliferation-inducing gene 46 protein; CK-18; CYK18; cytokeratin 18; K18; keratin 18, type I; keratin, type I cytoskeletal 18

Mouse

IgG1

Keratin, type I cytoskeletal 18

DE-K18 is a Mouse monoclonal IgG1, k antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 Mouse immunized with a cytoskeletal preparation extracted from the Human vulvar squamous carcinoma cell line A431.

Canine, Feline, Human, Zebra Fish

Canine;Feline;Human;Zebrafish

12352203