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ProductsBack/Mouse anti Human CD23

Mouse anti Human CD23

0231
Nordic-MUbio
ApplicationsFlow Cytometry
Product group Antibodies
TargetFCER2
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human CD23
  • Delivery Days Customer
    7
  • Application Supplier Note
    PBMC: Add10 microl of MAB/106 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2o to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20oC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow Cytometry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    LVNC-4
  • Conjugate
    Unconjugated
  • Gene ID2208
  • Target name
    FCER2
  • Target description
    Fc fragment of IgE receptor II
  • Target synonyms
    BLAST-2; CD23; CD23 antigen; CD23A; CLEC4J; C-type lectin domain family 4, member J; Fc epsilon receptor II; Fc fragment of IgE, low affinity II, receptor for (CD23); FCE2; fc-epsilon-RII; IGEBF; immunoglobulin E-binding factor; immunoglobulin epsilon-chain; low affinity immunoglobulin epsilon Fc receptor; lymphocyte IgE receptor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP06734
  • Protein Name
    Low affinity immunoglobulin epsilon Fc receptor
  • Shelf life instruction
    See expiration date on vial
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD23 using anti-CD23 antibody (A04237). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD23 antigen affinity purified polyclonal antibody (Catalog # A04237) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD23 at approximately 36KD. The expected band size for CD23 is at 36KD.
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