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ProductsBack/Mouse Anti-Human CD33-AF647

Mouse Anti-Human CD33-AF647

9590-31
SouthernBiotech
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman
TargetCD33
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Overview

  • Supplier
    SouthernBiotech
  • Product Name
    Mouse Anti-Human CD33-AF647
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    WM53
  • Conjugate
    AlexaFluor 647
  • Gene ID945
  • Target name
    CD33
  • Target description
    CD33 molecule
  • Target synonyms
    CD33rSiglec, SIGLEC-3, SIGLEC3, p67, myeloid cell surface antigen CD33, CD33 antigen (gp67), CD33 molecule transcript, gp67, sialic acid-binding Ig-like lectin 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP20138
  • Protein Name
    Myeloid cell surface antigen CD33
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of CD33 using anti-CD33 antibody (PB9932). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SKOV3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD33 antigen affinity purified polyclonal antibody (Catalog # PB9932) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD33 at approximately 45 kDa. The expected band size for CD33 is at 40 kDa.
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