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ProductsBack/MOUSE ANTI HUMAN TdT:FITC

MOUSE ANTI HUMAN TdT:FITC

MBS239317
MyBioSource
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Product group Antibodies
Price on request
0.025 mg
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Overview

  • Supplier
    MyBioSource
  • Product Name
    MOUSE ANTI HUMAN TdT:FITC
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Clone ID
    HT-1, HT-4, HT-8, HT-9
  • Concentration
    50.0 ug/ml
  • Isotype
    IgG
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TdT using anti-TdT antibody (PB9162). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TDT protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TdT antigen affinity purified polyclonal antibody (Catalog # PB9162) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TdT at approximately 39 kDa. The expected band size for TdT is at 39 kDa.
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