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ProductsBack/Mouse anti Human Tissue Inhibitor of Matrix Metalloproteinase 2 (TIMP2)

Mouse anti Human Tissue Inhibitor of Matrix Metalloproteinase 2 (TIMP2)

X2061M
Nordic-MUbio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityChicken, Human, Mouse, Porcine, Rabbit, Zebra Fish
TargetTIMP2

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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human Tissue Inhibitor of Matrix Metalloproteinase 2 (TIMP2)
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for ELISA, Western blotting (1-2 microg/ml) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 microg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Western Blotting;ELISA;Western Blotting;Immunohistochemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3A4
  • Conjugate
    Unconjugated
  • Gene ID7077
  • Target name
    TIMP2
  • Target description
    TIMP metallopeptidase inhibitor 2
  • Target synonyms
    CSC-21K, DDC8, metalloproteinase inhibitor 2, testicular secretory protein Li 57, tissue inhibitor of metalloproteinases 2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP16035
  • Protein Name
    Metalloproteinase inhibitor 2
  • Scientific Description
    CSC-21K; Metalloproteinase Inhibitor 2 precursor
  • Shelf life instruction
    See expiration date on vial
  • Reactivity
    Chicken, Human, Mouse, Porcine, Rabbit, Zebra Fish
  • Reactivity Supplier
    Human;Mouse;Rabbit;Chicken;Zebrafish;Pig
  • Reactivity Supplier Note
    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human TIMP-2 protein and mouse myeloma Ag8563 cells. Sequence common in mouse, rabbit, chicken, zebrafish and pig.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TIMP2 using anti-TIMP2 antibody (PB9830). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Spleen Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: Mouse Brain Tissue Lysate at 50ug, Lane 4: Mouse Thymus Tissue Lysate at 50ug, Lane 5: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP2 antigen affinity purified polyclonal antibody (Catalog # PB9830) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP2 at approximately 24 kDa. The expected band size for TIMP2 is at 24 kDa.
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