Mouse CD8a/Ly-2 Antibody : PE/CY5.5 (OASB00298)

Aviva Systems Biology

Mouse CD8a/Ly-2 Antibody : PE/CY5.5 (OASB00298)

23

Flow Cytometry

Research Use Only

Monoclonal

53-6.7

0.1 mg/ml

Double Conjugated

Liquid PBS/sodium azide and a stabilizing agent

Cd8a

CD8 subunit alpha

Ly-2, Ly-35, Ly-B, Lyt-2, T-cell surface glycoprotein CD8 alpha chain, CD8 antigen, alpha chain, Lyt-2.1 lymphocyte differentiation antigen (AA at 100), T-cell surface glycoprotein Lyt-2

Rat

IgG2a

In the mouse, CD8 exists in two forms - (i) a CD8 heterodimer composed of an alpha chain (CD8 alpha) and a beta chain (CD8 beta); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the suppressor/cytotoxic subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 alpha without CD8 beta. It has been suggested that CD8+ beta- T cells mature extrathymically, while development of the CD8 alpha+ beta+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8 alpha+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

2°C to 8°C

12352203