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ProductsBack/Mouse TNF-a Antibody : LE/AF (OASB01274)

Mouse TNF-a Antibody : LE/AF (OASB01274)

OASB01274
Aviva Systems Biology
ApplicationsFlow Cytometry, Western Blot, ELISpot Assay, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTnf
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    Mouse TNF-a Antibody : LE/AF (OASB01274)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ELISpot Assay, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MP6-XT3
  • Concentration
    0.5 mg/ml
  • Gene ID21926
  • Target name
    Tnf
  • Target description
    tumor necrosis factor
  • Target synonyms
    DIF, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tnfa, Tnfsf1a, Tnlg1f, tumor necrosis factor, cachectin, tumor necrosis factor ligand 1f, tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
  • Host
    Rat
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 25KD. The expected band size for TNF alpha is at 25KD.
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