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Western blot All lanes: MPZ antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 28, 35 kDa Observed band size: 28 kDa
Western blot All lanes: MPZ antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 28, 35 kDa Observed band size: 28 kDa
Western blot All lanes: MPZ antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 28, 35 kDa Observed band size: 28 kDa

MPZ Antibody

CSB-PA07844A0RB
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMPZ
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Overview

  • Supplier
    Cusabio
  • Product Name
    MPZ Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4359
  • Target name
    MPZ
  • Target description
    myelin protein zero
  • Target synonyms
    Charcot-Marie-Tooth neuropathy 1B; CHM; CHN2; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; CMTDID; DSS; HMSNIB; MPP; myelin peripheral protein; myelin protein P0; P0
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25189
  • Protein Name
    Myelin protein P0
  • Scientific Description
    Creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Myelin Protein Zero using anti-Myelin Protein Zero antibody (A00997-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myelin Protein Zero antigen affinity purified polyclonal antibody (Catalog # A00997-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myelin Protein Zero at approximately 28KD. The expected band size for Myelin Protein Zero is at 28KD.
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