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Bone lysates probed with Myelin Protein Zero Polyclonal Antibody, Unconjugated (bs-0337R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Bone lysates probed with Myelin Protein Zero Polyclonal Antibody, Unconjugated (bs-0337R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Bone lysates probed with Myelin Protein Zero Polyclonal Antibody, Unconjugated (bs-0337R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Myelin Protein Zero Polyclonal Antibody

BS-0337R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMPZ
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Overview

  • Supplier
    Bioss
  • Product Name
    Myelin Protein Zero Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4359
  • Target name
    MPZ
  • Target description
    myelin protein zero
  • Target synonyms
    CHM, CHN2, CMT1, CMT1B, CMT2I, CMT2J, CMT4E, CMTDI3, CMTDID, DSS, HMSNIB, MPP, P0, myelin protein P0, Charcot-Marie-Tooth neuropathy 1B, myelin peripheral protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25189
  • Protein Name
    Myelin protein P0
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Sodium-dependent Vitamin C transporter 2 deficiency impairs myelination and remyelination after injury: Roles of collagen and demethylation. Roehr D et al., 2017 Jul, Glia
    Read more
  • Recurrent laryngeal nerve regeneration using an oriented collagen scaffold containing Schwann cells. Chitose SI et al., 2017 Jul, Laryngoscope
    Read more
  • The importance of nerve microenvironment for schwannoma development. Schulz A et al., 2016 Aug, Acta Neuropathol
    Read more

Datasheet

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Figure 1. Western blot analysis of Myelin Protein Zero using anti-Myelin Protein Zero antibody (A00997-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myelin Protein Zero antigen affinity purified polyclonal antibody (Catalog # A00997-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myelin Protein Zero at approximately 28KD. The expected band size for Myelin Protein Zero is at 28KD.
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