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MPZ / P0 Antibody

LS-C331636
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMPZ
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MPZ / P0 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 27kDa/34kDa, while the observed MW by Western blot was 30kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:100), WB (1:500 - 1:2000) The predicted MW is 27kDa/34kDa, while the observed MW by Western blot was 30kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4359
  • Target name
    MPZ
  • Target description
    myelin protein zero
  • Target synonyms
    Charcot-Marie-Tooth neuropathy 1B; CHM; CHN2; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; CMTDID; DSS; HMSNIB; MPP; myelin peripheral protein; myelin protein P0; P0
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Myelin Protein Zero using anti-Myelin Protein Zero antibody (A00997-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myelin Protein Zero antigen affinity purified polyclonal antibody (Catalog # A00997-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myelin Protein Zero at approximately 28KD. The expected band size for Myelin Protein Zero is at 28KD.
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