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Western Blot analysis of 3T3 cells using CD206 Polyclonal Antibody
Western Blot analysis of 3T3 cells using CD206 Polyclonal Antibody
Western Blot analysis of 3T3 cells using CD206 Polyclonal Antibody

MRC1 Antibody

CSB-PA006303
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMRC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MRC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4360
  • Target name
    MRC1
  • Target description
    mannose receptor C-type 1
  • Target synonyms
    CD206, CLEC13D, CLEC13DL, MMR, MRC1L1, bA541I19.1, hMR, macrophage mannose receptor 1, C-type lectin domain family 13 member D, human mannose receptor, macrophage mannose receptor 1-like protein 1, mannose receptor, C type 1-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22897
  • Protein Name
    Macrophage mannose receptor 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MRC1 using anti-MRC1 antibody (A02285-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: human liver tissue lysates, Lane 4: monkey lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRC1 antigen affinity purified polyclonal antibody (Catalog # A02285-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRC1 at approximately 190-200 kDa. The expected band size for MRC1 is at 166 kDa.
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HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Antibody（bs-4727R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
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