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HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Antibody（bs-4727R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Antibody（bs-4727R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Antibody（bs-4727R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

MRC1 Polyclonal Antibody

BS-4727R
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetMRC1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MRC1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4360
  • Target name
    MRC1
  • Target description
    mannose receptor C-type 1
  • Target synonyms
    bA541I19.1; CD206; CLEC13D; CLEC13DL; C-type lectin domain family 13 member D; hMR; human mannose receptor; macrophage mannose receptor 1; macrophage mannose receptor 1-like protein 1; mannose receptor, C type 1-like 1; MMR; MRC1L1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22897
  • Protein Name
    Macrophage mannose receptor 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Sonodynamic therapy in atherosclerosis by curcumin nanosuspensions: Preparation design, efficacy evaluation, and mechanisms analysis. Jiang L et al., 2020 Jan, Eur J Pharm Biopharm
    Read more
  • Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata Michx fruit. Zhan Q et al., 2020 Feb 15, Int J Biol Macromol
    Read more
  • A Multi-Functional Silicon Nanoparticle Designed for Enhanced Osteoblast Calcification and Related Combination Therapy. Shao N et al., 2019 Dec, Macromol Biosci
    Read more
  • The effect of exposure to hypoxia on superoxide formation by alveolar macrophages is indirect. Zaloudikova M et al., 2019 Nov 1, Life Sci
    Read more
  • Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively Activated Macrophages in Mice. Nishikawa S et al., 2019, J Nutr Sci Vitaminol (Tokyo)
    Read more
  • Exploring the interactions between engineered nanomaterials and immune cells at 3D nano-bio interfaces to discover potent nano-adjuvants. Ma R et al., 2019 Jun 18, Nanomedicine
    Read more
  • Heat injured stromal cells-derived exosomal EGFR enhances prostatic wound healing after thulium laser resection through EMT and NF-kappaB signaling. Shi F et al., 2019 Aug, Prostate
    Read more
  • GLUT12 promotes prostate cancer cell growth and is regulated by androgens and CaMKK2 signaling. White MA et al., 2018 Apr, Endocr Relat Cancer
    Read more
  • Highly Dispersible and Bioavailable Curcumin but not Native Curcumin Induces Brown-Like Adipocyte Formation in Mice. Nishikawa S et al., 2018 Mar, Mol Nutr Food Res
    Read more
  • IL-17A induces heterogeneous macrophages, and it does not alter the effects of lipopolysaccharides on macrophage activation in the skin of mice. Nakai K et al., 2017 Sep 29, Sci Rep
    Read more

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Figure 1. Western blot analysis of MRC1 using anti-MRC1 antibody (A02285-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: human liver tissue lysates, Lane 4: monkey lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRC1 antigen affinity purified polyclonal antibody (Catalog # A02285-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRC1 at approximately 190-200 kDa. The expected band size for MRC1 is at 166 kDa.
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