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ProductsBack/MRP1 antibody [MRPr1]

MRP1 antibody [MRPr1]

GTX50894
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetABCC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    MRP1 antibody [MRPr1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    For FACS: Cell permeabilization required. Suggested starting dilution is 1:20. Optimal conditions must be determined individually for each application.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MRPr1
  • Conjugate
    Unconjugated
  • Gene ID4363
  • Target name
    ABCC1
  • Target description
    ATP binding cassette subfamily C member 1 (ABCC1 blood group)
  • Target synonyms
    ABC29, ABCC, DFNA77, GS-X, MRP, MRP1, multidrug resistance-associated protein 1, ATP-binding cassette transporter variant ABCC1delta-ex13&14, ATP-binding cassette transporter variant ABCC1delta-ex25&26, ATP-binding cassette, sub-family C (CFTR/MRP), member 1, LTC4 transporter, glutathione S-conjugate export pump, glutathione-S-conjugate-translocating ATPase ABCC1, leukotriene C(4) transporter
  • Host
    Rat
  • Isotype
    IgG2a
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Mairinger S, Sake JA, Hernández Lozano I, et al. Assessing the Activity of Multidrug Resistance-Associated Protein 1 at the Lung Epithelial Barrier. J Nucl Med. 2020,61(11):1650-1657. doi: 10.2967/jnumed.120.244038
    Read this paper

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Figure 1. Western blot analysis of MRP1/ABCC1 using anti-MRP1/ABCC1 antibody (A00872-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP1/ABCC1 antigen affinity purified polyclonal antibody (Catalog # A00872-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP1/ABCC1 at approximately 220 kDa. The expected band size for MRP1/ABCC1 is at 220 kDa.
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