Immunofluorescence analysis of methanol-fixed HeLa, using MTHFD2(GTX115482) antibody at 1:500 dilution.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115482/GTX115482_40457_20181228_WB_shRNA_watermark_w_23060519_591.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115482/GTX115482_40457_20190118_WB_R_lung_w_23060519_567.webp "Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115482/GTX115482_40457_20190118_WB_M_pancreas_w_23060519_319.webp "Whole cell extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![MTHFD2 antibody [N3C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. MTHFD2 stained by MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX115482/GTX115482_40457_20210226_IHC-P_w_23060519_412.webp "MTHFD2 antibody [N3C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. MTHFD2 stained by MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115482/GTX115482_40457_20190118_WB_w_23060519_794.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![MTHFD2 antibody [N3C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human glioblastoma. MTHFD2 stained by MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX115482/GTX115482_40457_20230825_IHC-P_23090619_115.webp "MTHFD2 antibody [N3C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human glioblastoma. MTHFD2 stained by MTHFD2 antibody [N3C3] (GTX115482) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Immunofluorescence analysis of methanol-fixed HeLa, using MTHFD2(GTX115482) antibody at 1:500 dilution.
MTHFD2 antibody [N3C3]
GTX115482
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMTHFD2
Overview
- SupplierGeneTex
- Product NameMTHFD2 antibody [N3C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.61 mg/ml
- ConjugateUnconjugated
- Gene ID10797
- Target nameMTHFD2
- Target descriptionmethylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
- Target synonymsNMDMC, bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase
- HostRabbit
- IsotypeIgG
- Protein IDP13995
- Protein NameBifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
- Scientific DescriptionThis gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chan CH, Wu CY, Dubey NK, et al. Modulating redox homeostasis and cellular reprogramming through inhibited methylenetetrahydrofolate dehydrogenase 2 enzymatic activities in lung cancer. Aging (Albany NY). 2020,12(18):17930-17947. doi: 10.18632/aging.103471Read this paper
- Selcuklu SD, Donoghue MT, Rehmet K, et al. MicroRNA-9 inhibition of cell proliferation and identification of novel miR-9 targets by transcriptome profiling in breast cancer cells. J Biol Chem. 2012,287(35):29516-28. doi: 10.1074/jbc.M111.335943Read this paper
Datasheet
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![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104990/GTX104990_44727_20220715_WB_23032819_842.webp)
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