![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101557/GTX101557_43957_20230428_WB_R_23050223_481.webp)
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
mTOR antibody [C3], C-term
GTX101557
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMTOR
Overview
- SupplierGeneTex
- Product NamemTOR antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.05 mg/ml
- ConjugateUnconjugated
- Gene ID2475
- Target nameMTOR
- Target descriptionmechanistic target of rapamycin kinase
- Target synonymsFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS, serine/threonine-protein kinase mTOR, FK506 binding protein 12-rapamycin associated protein 2, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, FKBP12-rapamycin complex-associated protein 1, mammalian target of rapamycin, mechanistic target of rapamycin (serine/threonine kinase), rapamycin and FKBP12 target 1, rapamycin associated protein FRAP2, rapamycin target protein 1, tyrosine-protein kinase mTOR
- HostRabbit
- IsotypeIgG
- Protein IDP42345
- Protein NameSerine/threonine-protein kinase mTOR
- Scientific DescriptionThe protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101557/GTX101557_41059_20150206_IHC_M_2_w_23060100_848.webp)



![mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101557/GTX101557_42018_20150814_IHC-P_M_w_23060100_952.webp)

![mTOR antibody [C3], C-term detects mTOR protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: mTOR stained by mTOR antibody [C3], C-term (GTX101557) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar= 10 μm. mTOR antibody [C3], C-term detects mTOR protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: mTOR stained by mTOR antibody [C3], C-term (GTX101557) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar= 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101557/GTX101557_41731_20190306_ICC_IF_w_23060100_682.webp)

![Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg of mTOR antibody [C3], C-term (GTX101557). Western blot analysis was performed using mTOR antibody [C3], C-term (GTX101557). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg of mTOR antibody [C3], C-term (GTX101557). Western blot analysis was performed using mTOR antibody [C3], C-term (GTX101557). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101557/GTX101557_41731_20150910_IP_w_23060100_263.webp)







