![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT649] (GTX630198) diluted at 1:500. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT649] (GTX630198) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630198/GTX630198_41512_20160616_WB_shRNA_watermark_w_23061202_784.webp)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT649] (GTX630198) diluted at 1:500.
mTOR antibody [GT649]
GTX630198
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetMTOR
Overview
- SupplierGeneTex
- Product NamemTOR antibody [GT649]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT649
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2475
- Target nameMTOR
- Target descriptionmechanistic target of rapamycin kinase
- Target synonymsFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS, serine/threonine-protein kinase mTOR, FK506 binding protein 12-rapamycin associated protein 2, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, FKBP12-rapamycin complex-associated protein 1, mammalian target of rapamycin, mechanistic target of rapamycin (serine/threonine kinase), rapamycin and FKBP12 target 1, rapamycin associated protein FRAP2, rapamycin target protein 1, tyrosine-protein kinase mTOR
- HostMouse
- IsotypeIgG1
- Protein IDP42345
- Protein NameSerine/threonine-protein kinase mTOR
- Scientific DescriptionThe protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Franco-Iborra S, Plaza-Zabala A, Montpeyo M, et al. Mutant HTT (huntingtin) impairs mitophagy in a cellular model of Huntington disease. Autophagy. 2021,17(3):672-689. doi: 10.1080/15548627.2020.1728096Read this paper
![mTOR antibody [GT649] detects mTOR protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [GT649] (GTX630198) dilution: 1:1000 mTOR antibody [GT649] detects mTOR protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [GT649] (GTX630198) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX630198/GTX630198_41512_WB_2_w_23061202_254.webp)
![mTOR antibody [GT649] detects mTOR protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. mTOR antibody [GT649] (GTX630198) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min mTOR antibody [GT649] detects mTOR protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. mTOR antibody [GT649] (GTX630198) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX630198/GTX630198_41512_20160427_IHC-P_M_w_23061202_262.webp)
![mTOR antibody [GT649] detects mTOR protein by western blot analysis. A Jurkat BRaji whole cell lysate/extract C NCI-H929 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [GT649] (GTX630198) dilution: 1:1000 mTOR antibody [GT649] detects mTOR protein by western blot analysis. A Jurkat BRaji whole cell lysate/extract C NCI-H929 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [GT649] (GTX630198) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX630198/GTX630198_41512_WB_w_23061202_621.webp)




![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101557/GTX101557_43957_20230428_WB_R_23050223_481.webp)
![mTOR antibody [C1], Internal detects mTOR protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal (GTX101558) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101558/GTX101558_39721_20151015_WB_R_brain_w_23060100_962.webp)
![mTOR antibody [GT6611] detects mTOR protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [GT6611] (GTX630199) dilution: 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX630199/GTX630199_41512_WB_2_w_23061202_438.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT778] (GTX630200) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630200/GTX630200_41512_20160616_WB_shRNA_watermark_w_23061202_703.webp)