More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/MURF1 antibody
IHC-P analysis of human skeletal muscle using GTX24125 MURF1 antibody. It shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section. Antigen retrieval : citrate buffer pH 6 Dilution : 1.25μg/ml
IHC-P analysis of human skeletal muscle using GTX24125 MURF1 antibody. It shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section. Antigen retrieval : citrate buffer pH 6 Dilution : 1.25μg/ml
IHC-P analysis of human skeletal muscle using GTX24125 MURF1 antibody. It shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section. Antigen retrieval : citrate buffer pH 6 Dilution : 1.25μg/ml

MURF1 antibody

GTX24125
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat, Zebra Fish
TargetTRIM63
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    MURF1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:100 - 1:2000. IHC-P: 1.25microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID84676
  • Target name
    TRIM63
  • Target description
    tripartite motif containing 63
  • Target synonyms
    CMH31, IRF, MURF1, MURF2, RNF28, SMRZ, E3 ubiquitin-protein ligase TRIM63, RING-type E3 ubiquitin transferase TRIM63, iris ring finger protein, muscle specific ring finger protein 2, muscle-specific RING finger protein 1, ring finger protein 28, striated muscle RING zinc finger protein, tripartite motif containing 63, E3 ubiquitin protein ligase, tripartite motif-containing protein 63
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ969Q1
  • Protein Name
    E3 ubiquitin-protein ligase TRIM63
  • Scientific Description
    This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012]
  • Reactivity
    Human, Rat, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Knee osteoarthritis induces atrophy and neuromuscular junction remodeling in the quadriceps and tibialis anterior muscles of rats. Cunha JE et al., 2019 Apr 24, Sci Rep
    Read this paper
  • Denervation causes fiber atrophy and myosin heavy chain co-expression in senescent skeletal muscle. Rowan SL et al., 2012, PLoS One
    Read this paper
  • The zebrafish runzel muscular dystrophy is linked to the titin gene. Steffen LS et al., 2007 Sep 15, Dev Biol
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
TRIM63 AntibodyCAB3101
ReactivityHuman
TargetTRIM63
  • SizePrice
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA168065(TRIM63 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)
Product group Antibodies
Cusabio
TRIM63 AntibodyCSB-PA168065
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTRIM63
  • SizePrice
Figure 1. Western blot analysis of MURF1/TRIM63 using anti-MURF1/TRIM63 antibody (A02016-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MURF1/TRIM63 antigen affinity purified polyclonal antibody (Catalog # A02016-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MURF1/TRIM63 at approximately 40KD. The expected band size for MURF1/TRIM63 is at 40KD.
Product group Antibodies
Boster Bio
Anti-MURF1/TRIM63 Antibody Picoband(r)A02016-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Monkey
TargetTRIM63
  • SizePrice
Anti-TRIM63 Antibody
Product group Antibodies
Antibodies.com
Anti-TRIM63 AntibodyA30694
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-MURF1 / TRIM63 (N Term)EB05879
ApplicationsELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human
TargetTRIM63
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MURF1 / IRF AntibodyLS-C402695
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetTRIM63
  • SizePrice
Product group Antibodies
MedChem Express
MuRF1 Antibody (YA4416, Mouse)HY-P84719
TargetTRIM63
  • SizePrice
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MuRF1 Polyclonal Antibody(bs-2539R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
MuRF1 Polyclonal AntibodyBS-2539R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetTRIM63
  • SizePrice
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody [HL1450] (GTX636922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
MURF1 antibody [HL1450]GTX636922
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetTRIM63
  • SizePrice
WB analysis of rat heart tissue lysate using GTX33334 MURF1 antibody. Dilution : 1:1000 Loading : 25μg
Product group Antibodies
References
GeneTex
MURF1 antibodyGTX33334
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetTRIM63
  • SizePrice