![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Muscleblind-like 1 antibody [N1C2] (GTX113773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Muscleblind-like 1 antibody [N1C2] (GTX113773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113773/GTX113773_40198_20190201_WB_w_23060501_374.webp)
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Muscleblind-like 1 antibody [N1C2] (GTX113773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Muscleblind-like 1 antibody [N1C2]
GTX113773
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMBNL1
Overview
- SupplierGeneTex
- Product NameMuscleblind-like 1 antibody [N1C2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.45 mg/ml
- ConjugateUnconjugated
- Gene ID4154
- Target nameMBNL1
- Target descriptionmuscleblind like splicing regulator 1
- Target synonymsEXP, MBNL, muscleblind-like protein 1, muscleblind-like, triplet-expansion RNA-binding protein
- HostRabbit
- IsotypeIgG
- Protein IDQ9NR56
- Protein NameMuscleblind-like protein 1
- Scientific DescriptionMediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5-YGCU(U/G)Y-3consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Muscleblind-like 1 antibody [N1C2] detects Muscleblind-like 1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Muscleblind-like 1 stained by Muscleblind-like 1 antibody [N1C2] (GTX113773) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Muscleblind-like 1 antibody [N1C2] detects Muscleblind-like 1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Muscleblind-like 1 stained by Muscleblind-like 1 antibody [N1C2] (GTX113773) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX113773/GTX113773_40198_20181004_ICC_IF_R_w_23060501_933.webp)







