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Western Blot analysis of HepG2 cells using MYH Polyclonal Antibody
Western Blot analysis of HepG2 cells using MYH Polyclonal Antibody
Western Blot analysis of HepG2 cells using MYH Polyclonal Antibody

MUTYH Antibody

CSB-PA003342
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMUTYH
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Overview

  • Supplier
    Cusabio
  • Product Name
    MUTYH Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4595
  • Target name
    MUTYH
  • Target description
    mutY DNA glycosylase
  • Target synonyms
    MYH, adenine DNA glycosylase, A/G-specific adenine DNA glycosylase, mutY homolog, mutY-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UIF7
  • Protein Name
    Adenine DNA glycosylase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MUTYH using anti-MUTYH antibody (A01618-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUTYH antigen affinity purified polyclonal antibody (Catalog # A01618-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUTYH at approximately 60 kDa. The expected band size for MUTYH is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-MUTYH Antibody Picoband(r)A01618-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Rat
TargetMUTYH
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Product group Antibodies
RayBiotech
Anti-MUTYH Antibody101-11043
ApplicationsImmunoFluorescence, Western Blot, ELISA
TargetMUTYH
  • SizePrice
Product group Antibodies
Biomatik
MutY Homolog (MUTYH) Polyclonal AntibodyCAU21660
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetMUTYH
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Product group Antibodies
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DNTTIP2 AntibodyCAB16120
ReactivityHuman
TargetMUTYH
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Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-MUTYH AntibodyHPA008732
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetMUTYH
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Product group Antibodies
Everest Biotech
Goat anti-MUTYH AntibodyEB06329
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetMUTYH
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Product group Antibodies
LifeSpan BioSciences
MUTYH / MYH AntibodyLS-C400901
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMUTYH
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MUTYH antibody [N3C3] (GTX103185) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
GeneTex
MUTYH antibody [N3C3]GTX103185
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetMUTYH
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Anti-MUTYH Antibody
Product group Antibodies
Antibodies.com
Anti-MUTYH AntibodyA96045
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
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