Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MUTYH antibody [N3C3] (GTX103185) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MUTYH antibody [N3C3] (GTX103185) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103185/GTX103185_40037_20210924_WB_w_23060119_153.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MUTYH antibody [N3C3] (GTX103185) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![MUTYH antibody [N3C3] detects MUTYH protein at mitochondria and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MUTYH protein stained by MUTYH antibody [N3C3] (GTX103185) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX103185/GTX103185_40037_20160224_IFA_w_23060119_393.webp "MUTYH antibody [N3C3] detects MUTYH protein at mitochondria and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MUTYH protein stained by MUTYH antibody [N3C3] (GTX103185) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MUTYH antibody [N3C3] (GTX103185) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
MUTYH antibody [N3C3]
GTX103185
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMUTYH
Overview
- SupplierGeneTex
- Product NameMUTYH antibody [N3C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.16 mg/ml
- ConjugateUnconjugated
- Gene ID4595
- Target nameMUTYH
- Target descriptionmutY DNA glycosylase
- Target synonymsMYH, adenine DNA glycosylase, A/G-specific adenine DNA glycosylase, mutY homolog, mutY-like protein
- HostRabbit
- IsotypeIgG
- Protein IDQ9UIF7
- Protein NameAdenine DNA glycosylase
- Scientific DescriptionThis gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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