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The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA018595(MVP Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA018595(MVP Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA018595(MVP Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)

MVP Antibody

CSB-PA018595
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMVP
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Overview

  • Supplier
    Cusabio
  • Product Name
    MVP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9961
  • Target name
    MVP
  • Target description
    major vault protein
  • Target synonyms
    LRP, VAULT1, major vault protein, lung resistance-related protein, testicular secretory protein Li 30
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14764
  • Protein Name
    Major vault protein
  • Scientific Description
    This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MVP using anti-MVP antibody (A00642-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: human SGC-7901 whole cell lysates, Lane 6: human MDA-MB-231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVP antigen affinity purified polyclonal antibody (Catalog # A00642-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MVP at approximately 99KD. The expected band size for MVP is at 99KD.
Product group Antibodies
Boster Bio
Anti-MVP Antibody Picoband(r)A00642-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetMVP
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ReactivityHuman
TargetMVP
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TargetMVP
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MVP Polyclonal AntibodyCAC13838
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetMVP
  • SizePrice
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (LRP/MVP) polyclonal Antibody, Unconjugated (bs-0661R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Product group Antibodies
References
Bioss
LRP/MVP Polyclonal AntibodyBS-0661R
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Zebra Fish
TargetMVP
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Product group Antibodies
Assay Genie
RAB3D AntibodyCAB10390
ReactivityHuman
TargetMVP
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Western blot All lanes: MVP antibody at 2microg/ml + Rat lung tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 100 kDa Observed band size: 110 kDa
Product group Antibodies
Cusabio
MVP AntibodyCSB-PA015248LA01HU
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ReactivityHuman, Rat
TargetMVP
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Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
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Anti-MVP AntibodyHPA002321
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TargetMVP
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