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NANOG Polyclonal Antibody

RD70401A
Reddot Biotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetNanog
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    NANOG Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    430 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID71950
  • Target name
    Nanog
  • Target description
    Nanog homeobox
  • Target synonyms
    2410002E02Rik, ENK, Stm1, ecat4, homeobox protein NANOG, ES cell-associated protein 4, early embryo specific expression NK family, early embryo specific expression NK-type homeobox protein, homeobox transcription factor Nanog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ80Z64
  • Protein Name
    Homeobox protein NANOG
  • Scientific Description
    The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. - This is a NANOG Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Nanog using anti-Nanog antibody (A00153-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nanog antigen affinity purified polyclonal antibody (Catalog # A00153-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nanog at approximately 37 kDa. The expected band size for Nanog is at 37 kDa.
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