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NCLN Antibody

ORB1239390
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNCLN
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Overview

  • Supplier
    Biorbyt
  • Product Name
    NCLN Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    NCLN antibody can be used for detection of NCLN by Western blot at 0.5 microg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 microg/mL. For immunofluorescence start at 2.5 microg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.. NCLN antibody can be used for detection of NCLN by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. ELISA, IF, IHC-P, WB
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID56926
  • Target name
    NCLN
  • Target description
    nicalin
  • Target synonyms
    NET59, BOS complex subunit NCLN, nicalin homolog, nicastrin-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ969V3
  • Protein Name
    BOS complex subunit NCLN
  • Scientific Description
    NCLN Antibody
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NCLN using anti-NCLN antibody (A12284-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse pancreas tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCLN antigen affinity purified polyclonal antibody (Catalog # A12284-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCLN at approximately 63 kDa. The expected band size for NCLN is at 63 kDa.
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