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Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Spleen lysates probed with NCR1 Polyclonal Antibody, Unconjugated (bs-10027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Spleen lysates probed with NCR1 Polyclonal Antibody, Unconjugated (bs-10027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Spleen lysates probed with NCR1 Polyclonal Antibody, Unconjugated (bs-10027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

NCR1 Polyclonal Antibody

BS-10027R
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNCR1
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Overview

  • Supplier
    Bioss
  • Product Name
    NCR1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9437
  • Target name
    NCR1
  • Target description
    natural cytotoxicity triggering receptor 1
  • Target synonyms
    CD335, LY94, NK-p46, NKP46, natural cytotoxicity triggering receptor 1, NK cell-activating receptor, lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46), natural killer cell p46-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO76036
  • Protein Name
    Natural cytotoxicity triggering receptor 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Pan P, Kang S, Wang Y, et al. Black Raspberries Enhance Natural Killer Cell Infiltration into the Colon and Suppress the Progression of Colorectal Cancer. Front Immunol. 2017,8:997. doi: 10.3389/fimmu.2017.00997
    Read this paper
  • Jaime-Ramirez AC, Yu JG, Caserta E, et al. Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma. Mol Ther Oncolytics. 2017,5:87-96. doi: 10.1016/j.omto.2017.05.002
    Read this paper
  • Vindevogel E, Baert T, VAN Hoylandt A, et al. The Use of Toll-like Receptor 4 Agonist to Reshape the Immune Signature in Ovarian Cancer. Anticancer Res. 2016,36(11):5781-5792.
    Read this paper

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Figure 1. Western blot analysis of NCR1 using anti-NCR1 antibody (A01516-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCR1 antigen affinity purified polyclonal antibody (Catalog # A01516-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCR1 at approximately 36KD. The expected band size for NCR1 is at 34KD.
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