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ProductsBack/NCR1 Rabbit Polyclonal Antibody
WB Suggested Anti-NCR1 Antibody Titration: 1.0 ug/ml Positive Control: OVCAR-3 Whole CellNCR1 is strongly supported by BioGPS gene expression data to be expressed in Human OVCAR3 cells
WB Suggested Anti-NCR1 Antibody Titration: 1.0 ug/ml Positive Control: OVCAR-3 Whole CellNCR1 is strongly supported by BioGPS gene expression data to be expressed in Human OVCAR3 cells
WB Suggested Anti-NCR1 Antibody Titration: 1.0 ug/ml Positive Control: OVCAR-3 Whole CellNCR1 is strongly supported by BioGPS gene expression data to be expressed in Human OVCAR3 cells

NCR1 Rabbit Polyclonal Antibody

TA358499
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetNCR1
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    NCR1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID9437
  • Target name
    NCR1
  • Target description
    natural cytotoxicity triggering receptor 1
  • Target synonyms
    CD335; LY94; lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); natural cytotoxicity triggering receptor 1; natural killer cell p46-related protein; NK cell-activating receptor; NKP46; NK-p46
  • Host
    Rabbit
  • Protein IDO76036
  • Protein Name
    Natural cytotoxicity triggering receptor 1
  • Scientific Description
    NCR1 Antibody - C-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Spleen lysates probed with NCR1 Polyclonal Antibody, Unconjugated (bs-10027R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of NCR1 using anti-NCR1 antibody (A01516-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCR1 antigen affinity purified polyclonal antibody (Catalog # A01516-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCR1 at approximately 36KD. The expected band size for NCR1 is at 34KD.
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