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IHC-P analysis of human breast adenocarcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
IHC-P analysis of human breast adenocarcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
IHC-P analysis of human breast adenocarcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.

NEK6 antibody [5D7]

GTX84058
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNEK6
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Overview

  • Supplier
    GeneTex
  • Product Name
    NEK6 antibody [5D7]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5D7
  • Concentration
    0.75 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10783
  • Target name
    NEK6
  • Target description
    NIMA related kinase 6
  • Target synonyms
    SID6-1512, serine/threonine-protein kinase Nek6, NIMA (never in mitosis gene a)-related kinase 6, never in mitosis A-related kinase 6, nimA-related protein kinase 6, protein kinase SID6-1512, putative serine-threonine protein kinase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9HC98
  • Protein Name
    Serine/threonine-protein kinase Nek6
  • Scientific Description
    Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NEK6 using anti-NEK6 antibody (A03740-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HT-1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEK6 antigen affinity purified polyclonal antibody (Catalog # A03740-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEK6 at approximately 45 kDa. The expected band size for NEK6 is at 36-45 kDa.
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