IHC-P analysis of human breast adenocarcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![IHC-P analysis of human colon adenocarcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84058/GTX84058_2242_IHC-P_w_23061420_223.webp "IHC-P analysis of human colon adenocarcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human ovary adenocarcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84058/GTX84058_2243_IHC-P_w_23061420_685.webp "IHC-P analysis of human ovary adenocarcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human kidney carcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84058/GTX84058_2244_IHC-P_w_23061420_962.webp "IHC-P analysis of human kidney carcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human lung carcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84058/GTX84058_2245_IHC-P_w_23061420_281.webp "IHC-P analysis of human lung carcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human pancreas carcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84058/GTX84058_2246_IHC-P_w_23061420_859.webp "IHC-P analysis of human pancreas carcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human prostate carcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84058/GTX84058_2247_IHC-P_w_23061420_377.webp "IHC-P analysis of human prostate carcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human thyroid carcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84058/GTX84058_2248_IHC-P_w_23061420_332.webp "IHC-P analysis of human thyroid carcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![FACS analysis of HEK293T cells transfected with either NEK6 plasmid(Red) or empty vector control plasmid(Blue) using GTX84058 NEK6 antibody [5D7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84058/GTX84058_288_FACS_w_23061420_949.webp "FACS analysis of HEK293T cells transfected with either NEK6 plasmid(Red) or empty vector control plasmid(Blue) using GTX84058 NEK6 antibody [5D7].")
![WB analysis of HEK293T cells transfected with NEK6 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84058 NEK6 antibody [5D7]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84058/GTX84058_4101_WB_w_23061420_604.webp "WB analysis of HEK293T cells transfected with NEK6 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84058 NEK6 antibody [5D7]. Loading : 5 ug per lane")
IHC-P analysis of human breast adenocarcinoma tissue using GTX84058 NEK6 antibody [5D7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
NEK6 antibody [5D7]
GTX84058
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNEK6
Overview
- SupplierGeneTex
- Product NameNEK6 antibody [5D7]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:1000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5D7
- Concentration0.75 mg/ml
- ConjugateUnconjugated
- Gene ID10783
- Target nameNEK6
- Target descriptionNIMA related kinase 6
- Target synonymsnever in mitosis A-related kinase 6; NIMA (never in mitosis gene a)-related kinase 6; nimA-related protein kinase 6; protein kinase SID6-1512; putative serine-threonine protein kinase; serine/threonine-protein kinase Nek6; SID6-1512
- HostMouse
- IsotypeIgG1
- Protein IDQ9HC98
- Protein NameSerine/threonine-protein kinase Nek6
- Scientific DescriptionActivated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203