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IHC-P analysis of human brain, purkinje neurons tissue using GTX13387 NEK6 antibody. Antigen retrieval : Heat-induced antigen retrieval
IHC-P analysis of human brain, purkinje neurons tissue using GTX13387 NEK6 antibody. Antigen retrieval : Heat-induced antigen retrieval
IHC-P analysis of human brain, purkinje neurons tissue using GTX13387 NEK6 antibody. Antigen retrieval : Heat-induced antigen retrieval

NEK6 antibody

GTX13387
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNEK6
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Overview

  • Supplier
    GeneTex
  • Product Name
    NEK6 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 13 microg/ml. IHC-P: 13 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10783
  • Target name
    NEK6
  • Target description
    NIMA related kinase 6
  • Target synonyms
    SID6-1512, serine/threonine-protein kinase Nek6, NIMA (never in mitosis gene a)-related kinase 6, never in mitosis A-related kinase 6, nimA-related protein kinase 6, protein kinase SID6-1512, putative serine-threonine protein kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HC98
  • Protein Name
    Serine/threonine-protein kinase Nek6
  • Scientific Description
    The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NEK6 using anti-NEK6 antibody (A03740-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HT-1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEK6 antigen affinity purified polyclonal antibody (Catalog # A03740-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEK6 at approximately 45 kDa. The expected band size for NEK6 is at 36-45 kDa.
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