IHC-P analysis of breast adenocarcinoma tissue using GTX84056 NEK6 antibody [8G5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
![IHC-P analysis of kidney carcinoma tissue using GTX84056 NEK6 antibody [8G5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84056/GTX84056_2237_IHC-P_w_23061420_539.webp "IHC-P analysis of kidney carcinoma tissue using GTX84056 NEK6 antibody [8G5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of pancreas carcinoma tissue using GTX84056 NEK6 antibody [8G5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84056/GTX84056_2238_IHC-P_w_23061420_696.webp "IHC-P analysis of pancreas carcinoma tissue using GTX84056 NEK6 antibody [8G5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of endometrium tissue using GTX84056 NEK6 antibody [8G5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84056/GTX84056_2239_IHC-P_w_23061420_100.webp "IHC-P analysis of endometrium tissue using GTX84056 NEK6 antibody [8G5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of ovary tissue using GTX84056 NEK6 antibody [8G5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84056/GTX84056_2240_IHC-P_w_23061420_270.webp "IHC-P analysis of ovary tissue using GTX84056 NEK6 antibody [8G5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IP analysis of DDDDK tagged NEK6 overexpressed HEK293T lysate using GTX84056 NEK6 antibody [8G5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84056/GTX84056_3374_IP_w_23061420_758.webp "IP analysis of DDDDK tagged NEK6 overexpressed HEK293T lysate using GTX84056 NEK6 antibody [8G5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of HEK293T cells transfected with NEK6 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84056 NEK6 antibody [8G5]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84056/GTX84056_4100_WB_w_23061420_446.webp "WB analysis of HEK293T cells transfected with NEK6 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84056 NEK6 antibody [8G5]. Loading : 5 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with NEK6 plasmid using GTX84056 NEK6 antibody [8G5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84056/GTX84056_930_ICCIF_w_23061420_474.webp "ICC/IF analysis of COS7 cells transiently transfected with NEK6 plasmid using GTX84056 NEK6 antibody [8G5].")
IHC-P analysis of breast adenocarcinoma tissue using GTX84056 NEK6 antibody [8G5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
NEK6 antibody [8G5]
GTX84056
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNEK6
Overview
- SupplierGeneTex
- Product NameNEK6 antibody [8G5]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500. ICC/IF: 1:100. IHC-P: 1:50. IP: 2-4ug/mg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8G5
- Concentration0.74 mg/ml
- ConjugateUnconjugated
- Gene ID10783
- Target nameNEK6
- Target descriptionNIMA related kinase 6
- Target synonymsnever in mitosis A-related kinase 6; NIMA (never in mitosis gene a)-related kinase 6; nimA-related protein kinase 6; protein kinase SID6-1512; putative serine-threonine protein kinase; serine/threonine-protein kinase Nek6; SID6-1512
- HostMouse
- IsotypeIgG1
- Protein IDQ9HC98
- Protein NameSerine/threonine-protein kinase Nek6
- Scientific DescriptionActivated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203