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ProductsBack/Nesprin3 Recombinant Antbody Kit (KD-Validated)

Nesprin3 Recombinant Antbody Kit (KD-Validated)

BSM-90202R
Bioss Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
TargetSYNE3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Nesprin3 Recombinant Antbody Kit (KD-Validated)
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), IC(1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID161176
  • Target name
    SYNE3
  • Target description
    spectrin repeat containing nuclear envelope family member 3
  • Target synonyms
    C14orf139; C14orf49; KASH domain-containing protein 3; KASH3; LINC00341; long intergenic non-protein coding RNA 341; NCRNA00341; Nesp3; nesprin-3; NET53; nuclear envelope spectrin repeat protein 3; uncharacterized protein C14orf113
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZMZ3
  • Protein Name
    Nesprin-3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nesprin3/SYNE3 using anti-Nesprin3/SYNE3 antibody (A13164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nesprin3/SYNE3 antigen affinity purified polyclonal antibody (Catalog # A13164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nesprin3/SYNE3 at approximately 100 kDa. The expected band size for Nesprin3/SYNE3 is at 112 kDa.
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