IHC-P analysis of human kidney tissue using GTX30671 Nestin antibody [10C2]. Dilution : 1:500
![FACS (Intracellular staining) analysis of 1 x 10? CHO (A) and HEK293 (B) cells using GTX30671 Nestin antibody [10C2]. Blue : Primary antibody Orange : isotype control Dilution : 1 microg/10? cells](https://www.genetex.com/upload/website/prouct_img/normal/GTX30671/GTX30671_104_FACS_w_23060722_400.webp "FACS (Intracellular staining) analysis of 1 x 10? CHO (A) and HEK293 (B) cells using GTX30671 Nestin antibody [10C2]. Blue : Primary antibody Orange : isotype control Dilution : 1 microg/10? cells")
![WB analysis of (1) rat brain tissue, and (2) human CNS progenitor cell lysate using GTX30671 Nestin antibody [10C2]. The antibody did not react with Rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein. Loading : 5microg Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX30671/GTX30671_1471_WB_w_23060722_518.webp "WB analysis of (1) rat brain tissue, and (2) human CNS progenitor cell lysate using GTX30671 Nestin antibody [10C2]. The antibody did not react with Rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein. Loading : 5microg Dilution : 1:1000")
![ICC/IF analysis of F9 cells using GTX30671 Nestin antibody [10C2]. Green : primary antibody(left) and isotype control(right) Blue : DAPI Dilution : 1:50 Permibilization : 0.1% Triton X-100](https://www.genetex.com/upload/website/prouct_img/normal/GTX30671/GTX30671_234_ICC-IF_w_23060722_167.webp "ICC/IF analysis of F9 cells using GTX30671 Nestin antibody [10C2]. Green : primary antibody(left) and isotype control(right) Blue : DAPI Dilution : 1:50 Permibilization : 0.1% Triton X-100")
IHC-P analysis of human kidney tissue using GTX30671 Nestin antibody [10C2]. Dilution : 1:500
Nestin antibody [10C2]
GTX30671
ApplicationsFlow Cytometry, ImmunoFluorescence, Mass Spectrometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHamster, Human, Mouse
TargetNES
Overview
- SupplierGeneTex
- Product NameNestin antibody [10C2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000 - 1:5000. ICC/IF: 1:50 - 1:200. IHC-P: 1:100 - 1:200. IHC-Fr: 1:100 - 1:200. FACS: 1 microg / 106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Mass Spectrometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID10C2
- Concentration2.4 mg/ml
- ConjugateUnconjugated
- Gene ID10763
- Target nameNES
- Target descriptionnestin
- Target synonymsNbla00170, nestin
- HostMouse
- IsotypeIgG1
- Protein IDP48681
- Protein NameNestin
- Scientific DescriptionNestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
- ReactivityHamster, Human, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Ávila-González D, Romero-Morales I, Caro L, et al. Increased proliferation and neuronal fate in prairie vole brain progenitor cells cultured in vitro: effects by social exposure and sexual dimorphism. Biol Sex Differ. 2023,14(1):77. doi: 10.1186/s13293-023-00563-2Read this paper
- Yang JL, Fan H, Fu FF, et al. Transient neurogenesis in ischemic cortex from Sox2(+) astrocytes. Neural Regen Res. 2023,18(7):1521-1526. doi: 10.4103/1673-5374.357910Read this paper
- Piña-Medina AG, Díaz NF, Molina-Hernández A, et al. Effects of progesterone on the cell number of gliomaspheres derived from human glioblastoma cell lines. Life Sci. 2020,249:117536. doi: 10.1016/j.lfs.2020.117536Read this paper
- Lu P, Ceto S, Wang Y, et al. Prolonged human neural stem cell maturation supports recovery in injured rodent CNS. J Clin Invest. 2017,127(9):3287-3299. doi: 10.1172/JCI92955Read this paper
- García-Castro IL, García-López G, Ávila-González D, et al. Markers of Pluripotency in Human Amniotic Epithelial Cells and Their Differentiation to Progenitor of Cortical Neurons. PLoS One. 2015,10(12):e0146082. doi: 10.1371/journal.pone.0146082Read this paper
Datasheet
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