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Nestin Antibody

HY-P81120
MedChem Express
TargetNES
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Nestin
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10763
  • Target name
    NES
  • Target description
    nestin
  • Target synonyms
    Nbla00170; nestin
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Nestin Antibody is an unconjugated, approximately 268 kDa, rabbit-derived, anti-Nestin monoclonal antibody. Nestin Antibody can be used for: WB, IF-Cell, IF-Tissue, IHC-P expriments in human background without labeling.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nestin using anti-Nestin antibody (PB9874). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nestin antigen affinity purified polyclonal antibody (Catalog # PB9874) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nestin at approximately 230 kDa. The expected band size for Nestin is at 177 kDa.
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