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IHC-P analysis of mouse brain tissue (dentate gyrus and subventricular zone) using GTX30773 NeuN antibody [2Q158]. Red : Primary antibody Green : BrdU
IHC-P analysis of mouse brain tissue (dentate gyrus and subventricular zone) using GTX30773 NeuN antibody [2Q158]. Red : Primary antibody Green : BrdU
IHC-P analysis of mouse brain tissue (dentate gyrus and subventricular zone) using GTX30773 NeuN antibody [2Q158]. Red : Primary antibody Green : BrdU

NeuN antibody [2Q158]

GTX30773
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRbfox3
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Overview

  • Supplier
    GeneTex
  • Product Name
    NeuN antibody [2Q158]
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:10-1:100. IHC-P: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2Q158
  • Conjugate
    Unconjugated
  • Gene ID52897
  • Target name
    Rbfox3
  • Target description
    RNA binding protein, fox-1 homolog (C. elegans) 3
  • Target synonyms
    fox-1 homolog C; Fox-3; hexaribonucleotide binding protein 3; Hrn; Hrnbp3; Ne; NeuN; neuN antigen; Neuna60; neuronal nuclear antigen A60; neuronal nuclei antigen; RNA binding protein fox-1 homolog 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ8BIF2
  • Protein Name
    RNA binding protein fox-1 homolog 3
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons.
    Read more
  • Thrombospondin-2 promotes the proliferation and migration of glioma cells and contributes to the progression of glioma.
    Read more
  • Ubiquitin ligase Triad1 promotes neurite outgrowth by inhibiting MDM2-mediated ubiquitination of the neuroprotective factor pleiotrophin.
    Read more
  • The Temporal and Spatial Changes of Th17, Tregs, and Related Cytokines in Epilepsy Lesions. Wei J et al., 2022, Appl Bionics Biomech
    Read more
  • miR-204-5p is sponged by TUG1 to aggravate neuron damage induced by focal cerebral ischemia and reperfusion injury through upregulating COX2. Xiang P et al., 2022 Feb 28, Cell Death Discov
    Read more
  • Reducing host aldose reductase activity promotes neuronal differentiation of transplanted neural stem cells at spinal cord injury sites and facilitates locomotion recovery. Zhang K et al., 2022 Aug, Neural Regen Res
    Read more
  • CTRP1 Attenuates Cerebral Ischemia/Reperfusion Injury via the PERK Signaling Pathway. Fei H et al., 2021, Front Cell Dev Biol
    Read more
  • Elevated miR-29a Contributes to Axonal Outgrowth and Neurological Recovery After Intracerebral Hemorrhage via Targeting PTEN/PI3K/Akt Pathway. Zhao M et al., 2021 Nov, Cell Mol Neurobiol
    Read more
  • 3,6-dithiopomalidomide reduces neural loss, inflammation, behavioral deficits in brain injury and microglial activation. Lin CT et al., 2020 Jun 26, Elife
    Read more
  • T1-11, an adenosine derivative, ameliorates aging-related behavioral physiology and senescence markers in aging mice. Hsu WH et al., 2020 Jun 5, Aging (Albany NY)
    Read more

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Figure 1. Western blot analysis of NeuN/Rbfox3 using anti-NeuN/Rbfox3 antibody (A11954-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NeuN/Rbfox3 antigen affinity purified polyclonal antibody (Catalog # A11954-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NeuN/Rbfox3 at approximately 46-55 kDa. The expected band size for NeuN/Rbfox3 is at 34 kDa.
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