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ProductsBack/NeuN antibody [2Q158]
IHC-P analysis of rat cerebellum tissue using GTX30773 NeuN antibody [2Q158].Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Antigen retireval : Citrate Buffer, pH 6.0 Dilution : 1:100
IHC-P analysis of rat cerebellum tissue using GTX30773 NeuN antibody [2Q158].Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Antigen retireval : Citrate Buffer, pH 6.0 Dilution : 1:100
IHC-P analysis of rat cerebellum tissue using GTX30773 NeuN antibody [2Q158].Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Antigen retireval : Citrate Buffer, pH 6.0 Dilution : 1:100

NeuN antibody [2Q158]

GTX30773
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Mouse, Rabbit, Reptile, Rat
TargetRbfox3
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Overview

  • Supplier
    GeneTex
  • Product Name
    NeuN antibody [2Q158]
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:10-1:100. IHC-P: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2Q158
  • Conjugate
    Unconjugated
  • Gene ID52897
  • Target name
    Rbfox3
  • Target description
    RNA binding protein, fox-1 homolog (C. elegans) 3
  • Target synonyms
    Fox-3, Hrnbp3, NeuN, Neuna60, RNA binding protein fox-1 homolog 3, fox-1 homolog C, hexaribonucleotide binding protein 3, neuN antigen, neuronal nuclear antigen A60, neuronal nuclei antigen
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ8BIF2
  • Protein Name
    RNA binding protein fox-1 homolog 3
  • Reactivity
    Canine, Human, Mouse, Rabbit, Reptile, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons.
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  • Thrombospondin-2 promotes the proliferation and migration of glioma cells and contributes to the progression of glioma.
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  • Ubiquitin ligase Triad1 promotes neurite outgrowth by inhibiting MDM2-mediated ubiquitination of the neuroprotective factor pleiotrophin.
    Read this paper
  • The Temporal and Spatial Changes of Th17, Tregs, and Related Cytokines in Epilepsy Lesions. Wei J et al., 2022, Appl Bionics Biomech
    Read this paper
  • miR-204-5p is sponged by TUG1 to aggravate neuron damage induced by focal cerebral ischemia and reperfusion injury through upregulating COX2. Xiang P et al., 2022 Feb 28, Cell Death Discov
    Read this paper
  • Reducing host aldose reductase activity promotes neuronal differentiation of transplanted neural stem cells at spinal cord injury sites and facilitates locomotion recovery. Zhang K et al., 2022 Aug, Neural Regen Res
    Read this paper
  • CTRP1 Attenuates Cerebral Ischemia/Reperfusion Injury via the PERK Signaling Pathway. Fei H et al., 2021, Front Cell Dev Biol
    Read this paper

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Figure 1. Western blot analysis of NeuN/Rbfox3 using anti-NeuN/Rbfox3 antibody (A11954-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NeuN/Rbfox3 antigen affinity purified polyclonal antibody (Catalog # A11954-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NeuN/Rbfox3 at approximately 46-55 kDa. The expected band size for NeuN/Rbfox3 is at 34 kDa.
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