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NeuN antibody

GTX133127
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
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Product group Antibodies
TargetRbfox3
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Overview

  • Supplier
    GeneTex
  • Product Name
    NeuN antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.42 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID52897
  • Target name
    Rbfox3
  • Target description
    RNA binding protein, fox-1 homolog (C. elegans) 3
  • Target synonyms
    fox-1 homolog C; Fox-3; hexaribonucleotide binding protein 3; Hrn; Hrnbp3; Ne; NeuN; neuN antigen; Neuna60; neuronal nuclear antigen A60; neuronal nuclei antigen; RNA binding protein fox-1 homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8BIF2
  • Protein Name
    RNA binding protein fox-1 homolog 3
  • Scientific Description
    RNA-binding protein that regulates alternative splicing events.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity. Ciarpella F et al., 2021 Dec 17, iScience
    Read more
  • Blockade of dengue virus infection and viral cytotoxicity in neuronal cells in vitro and in vivo by targeting endocytic pathways. Ho MR et al., 2017 Jul 31, Sci Rep
    Read more

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Figure 1. Western blot analysis of NeuN/Rbfox3 using anti-NeuN/Rbfox3 antibody (A11954-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NeuN/Rbfox3 antigen affinity purified polyclonal antibody (Catalog # A11954-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NeuN/Rbfox3 at approximately 46-55 kDa. The expected band size for NeuN/Rbfox3 is at 34 kDa.
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IHC-P analysis of mouse brain tissue (dentate gyrus and subventricular zone) using GTX30773 NeuN antibody [2Q158]. Red : Primary antibody Green : BrdU
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